|
KMID : 1094719970020010059
|
|
Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.59 ~ p.59
|
|
|
Biochemical analysis of BmNPV attachment toBombyx mori BmN-4 cells
|
|
Kim Tae-Yong
Kang Han-Chul
Park Jin-O
Park Jong-Hwa
Kang Seok-Kwon
Chung In-Sik
|
|
|
Abstract
|
|
|
|
Binding characteristics ofBombyx mori nuclear polyhedrosis virus (BmNPV) toB. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.
|
|
|
KEYWORD
|
|
|
Bombyx mori nuclear polyhedrosis virus, BmN-4 cells, binding site
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|